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Fast speeds, high precision, easy usage and absolute reliability united in a flexible concept – that is the Mastercycler pro. Today’s requirements for PCR in your lab are defined by today’s research project. Tomorrow, new questions are arising and new experiments have to give the answers. That is why only an open and flexible system fit into today’s lab. Eppendorf’s Mastercycler pro offers ultimate flexibility:
› choose from three block formats
› operate the Mastercycler pro in three different configurations
› use any plate, tube or strip you want
Unsurpassed speed can be achieved with Eppendorf’s Impulse PCR, a device driven hot-start function that increases heating rates to 8 °C/s.
Evaporation in a PCR reaction vessel not only limits the ability to work with low reaction volumes, but also makes it very difficult to obtain reproducible results. For the Mastercycler pro it has been Eppendorf’s aim to protect the PCR reaction from evaporation. While common PCR instruments press a metal plate onto the PCR consumables, the vapo.protect lid covers the PCR consumables with a cushion that fits to the shape of the consumables – no matter which consumables you use. The snug fit of the cushion, the high pressure of the lid and the excellent heating capabilities of the lid reduce evaporation to a minimum. Evaporation leads to increasing concentrations of the PCR master mix components, especially primers. This, in turn, leads to non-specific binding. Due to the ongoing evaporation, this increased concentration is even changing during the experiment and between experiments – a true nightmare for any scientist who tries to keep variables to a minimum.
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